Green mould disease caused by Penicillium halotolerans infecting locally cultivated Citrus latifolia in Iraq

Published
Oct 6, 2025Abstract
To identify the causal agent, a total of 8 symptomatic fruits were collected. The features were as follow: green-blue fungus growth on the fruitiness superficial, after that fruit decaying finally. The fungi were grown on Cazepek yeast extract agar, Malt extract media, and Glycerol nitrate agar, Incubation in different temperature (4, 25, and 37 ⁰C). Molecular identification of P. halotolerans was uitilized for the genomic DNA extraction by utilizing an extraction kit (Zymo Research environmental purification, Quick-DNA™ Miniprep Plus Kit). The sequences of isolate MBJ1 were deposited in GenBank (OR622875), which was first report of green mould disease cuased by P. halotolerans infecting Citrus latifolia in Iraq.
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