Quantitative Analysis and Antifungal Screening of Astragalin, Apiin, and Epicatechin Isolated from Nigella sativa, Cuminum cyminum, and Crataegus rhipidophylla via HPLC preparation
Published
Oct 6, 2025Pages
1-10Abstract
The study delves into the quantitative analysis and antifungal screening of Astragalin, Apiin, and Epicatechin extracted from Nigella sativa, Cuminum cyminum, and Crataegus rhipidophylla through High-Performance Liquid Chromatography (HPLC). These bioactive compounds, noted constituents of these botanical sources, exhibit substantial therapeutic potential and have drawn attention for their pharmacological applications. Leveraging the precision and sensitivity of HPLC, this research quantifies the concentrations of these compounds, aiming to underscore their pharmaceutical potential and therapeutic efficacy.
Beyond quantification, the investigation extends to assess the antifungal properties of these compounds against Candida albicans. The inhibitory effects of Astragalin, Apiin, and Epicatechin are evaluated, contributing novel insights into natural antifungal agents. This comprehensive approach intertwines quantitative HPLC analysis with antifungal screening, aiming to provide a holistic understanding of these botanical compounds' potency and medicinal applications.
Results indicate promising potential for pharmaceutical development in combatting fungal infections. Astragalin demonstrated the highest inhibitory activity, followed by Apiin, while Epicatechin exhibited lower efficacy possibly due to its lower concentration. Purification and isolation of these compounds via HPLC highlighted their concentrations and purity, further showcasing their potential as natural antifungal agents.
This research aims to bridge the gap between botanical sources and pharmaceutical applications, providing insights into the pharmacological potential of these natural compounds and their utility in combating fungal infections.
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