Evaluation of some bioremediation applications of Tannase enzyme purified from ascomycete fungus Helvella bachu

Section: Research Article
Issue
Vol. 3 No. 2 (2025): Journal of Rafidain Environment July 2025
Published
Oct 6, 2025
Pages
78-87

Abstract

         With the aim of reducing or stopping the threats to ecosystems, the current study was designed to investigate the ability of the tannase enzyme purified from the local isolate of ascomycete fungus Helvella bachu in reducing the most dangerous toxin aflatoxin B1, using high-performance liquid chromatography technique, aflatoxin B1concentration was reduced to 3.4 ng/ml. Tannase enzyme analyzed the aflatoxin into two compounds in terms of the appearance of two peaks at a retention time 2.08 minutes and 4.59 minutes, with a residual area percentage 13.8 and 86.6% respectively, compared to standard aflatoxin, which appeared with one peak at a retention time 4.79 minutes. The concentration of aflatoxin decreased to 1.2 ng/ml using the crude enzyme compared to aflatoxin before treatment 1.32 µg/ml at the same retention times for the purified enzyme with a difference in the remaining area 45.8 and 54.2% respectively. Moreover tannase enzyme analyzed the aflatoxin into two new compounds . The ability of tannase enzyme to  remove a number of industrial dyes was also evaluated in a period of time ranging from (6-48) hours, the results showed that the enzyme was able to remove 100% of the dyes: Reactive Yellow, Indigo Carmina, and Orange G. but with varying percentages of the other dyes used after 48 hours of enzyme treatment.


The aim of this study was investigate the ability of the purified tannase enzyme from the ascomycete fungus Helvella bachu to treat aflatoxin B1 and number of industrial dyes.

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Authors

Jehaan Mowafak Al-rawi
Shimal Younis Abdul-hadi

Identifiers

https://doi.org/10.33899/jre.v3i2.49939
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