Evaluating the biological effect of green manure extract in a plant tissue culture system

Section: Articles
Issue
Vol. 4 No. 2 (2026): Journal of Rafidain Environment
Pages
55-71

Abstract

    This study aimed to evaluate the effect of adding green manure and eggshell powder to a tissue culture system used for callus induction and growth in Portulaca oleracea. The experiment was conducted using MS medium prepared with different concentrations of (25, 50, 100) ml/L of green manure extract and (100, 200, 300) g/L of eggshell powder. Callus induction and growth were studied, in addition to measuring fresh and dry weight and protein percentage after (30) and (60) days of inoculation. The results showed that the best response to callus induction was in the treatment to which 100 ml/L of green manure extract and 300 g/L of eggshell powder were added, which led to an increase in the level of other vital indicators. The highest average fresh weight was (3.0, 4.0) at (30, 60) days of age, respectively. As for the protein percentage, its highest value was in the treatment to which 50 ml/L of green manure extract and 200 g/L of eggshell powder were added, reaching (14.08, 17.76) at (30, 60) days of age, respectively.

References

  1. Al-Bakr, R. A. (2018). Propagation of Portulaca oleracea L. and level of active compounds in callus culture. Int. J. Enh. Res. Sci. Technol. Eng, 7, 33-43.
  2. Al-Faki, W. A., & Alnoumesi, A. A. (2024). Nutritional and medicinal properties of Portulaca oleracea L. Journal of Medicinal Plants Studies, 12(3), 78–85.
  3. Chandran, S., Unni, M. R., Thomas, S., & Kannan, V. R. (2020). Plant tissue culture: A biotechnological tool for sustainable crop production. Biocatalysis and Agricultural Biotechnology, 23, 101489.
  4. Chen, Y., Liu, T., Wang, J., & Zhang, X. (2021). Green manure crops and their role in sustainable agricultural systems. Agriculture, Ecosystems & Environment, 315, 107435.
  5. Food and Agriculture Organization of the United Nations (FAO). (2022). The state of food and agriculture 2022: Leveraging automation in agriculture for transforming agrifood systems. FAO.
  6. Godfray, H. C. J., Mason-D’Croz, D., Robinson, S., et al. (2021). Innovation for sustainable agricultural intensification. Global Food Security, 30, 100582.
  7. Kholaf, G. M. (2025). Enhancement of food products with Portulaca oleracea: Nutritional evaluation. Egyptian Journal of Food Science, 53(1), 23–31
  8. Kim, J. H., Lee, S. Y., & Park, K. Y. (2021). Effect of organic fertilizer application on plant growth and soil chemical properties under different crops cultivation. Korean Journal of Soil Science and Fertilizer, 54(4), 643–650
  9. Kumar, P., Singh, R., & Verma, S. (2022). Green manure incorporation improves soil fertility and enzymatic activity in cropping systems. Soil Biology and Biochemistry, 165, 108541. https://doi.org/10.1016/j.soilbio.2022.
  10. Kumar, R., Bohra, J. S., Kumawat, N., & Singh, A. K. (2021). Green manuring: A potential tool for soil sustainability and crop productivity. Journal of Soil Science and Plant Nutrition, 21(2), 843–856.
  11. Li, Y., Zhang, H., Chen, X., & Liu, Q. (2023). Intercropping enhances plant growth and nutrient use efficiency through improved soil biological activity. Journal of Cleaner Agriculture, 12(2), 145–158.
  12. Long, Y., Wang, X., Li, J., & Zhang, S. (2022). Optimization of culture media components for efficient plant regeneration in vitro. Plant Cell, Tissue and Organ Culture, 149(1), 1–12.
  13. Mahima, S., Singh, R., & Sharma, P. (2024). Proximate composition and mineral analysis of eggshell powder for potential dietary supplementation. International Journal of Biological and Pharmaceutical Sciences, 15(5), 78–85.
  14. Mousa, A. M., Abd- ELShafy, E., Ramadan, B., Khafagi, A. and Abou Elkhier, Z. A. (2022). Callus induction and influence of biotic and abiotic elicitation on active constituents of Portulaca oleracea L. calli induced in vitro. Egypt. J. Chem.65: (11). 29 – 40
  15. Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. plant, 15, 473-497
  16. Narayanan, S., Patra, B., & Tuteja, N. (2024). Calcium (Ca²⁺) signaling in plants: A plant stress perspective. South African Journal of Botany, 169, 464–485.
  17. Reddy, A. S. N., Ali, G. S., & Celesnik, H. (2023). Calcium signaling in plants: Emerging roles and regulatory mechanisms. Plant Science, 330, 111–120
  18. Sekhar, A. C., Reddy, P. R., & Kumar, S. (2023a). Advances in plant tissue culture for sustainable crop production. Plant Biotechnology Reports, 17(2), 145–158.
  19. Sekhar, S., Patra, B., & Panda, S. K. (2023b). Role of plant biotechnology in sustainable agriculture and food security. Frontiers in Plant Science, 14, 1182456.
  20. Smith, P., Haberl, H., Popp, A., et al. (2012). How much land-based greenhouse gas mitigation can be achieved without compromising food security and environmental goals? Global Change Biology, 19(8), 2285–2302.
  21. Tamanna, S., Amin, M. R., Rahman, M. S., & Chowdhury, M. M. (2025). Chemical composition and nutritional potential of eggshell powder: A detailed analysis. Journal of Food Science and Technology, 60(4), 1123–1135.
  22. Tarkergari, K., Waghray, K., & Gulla, S. (2024). Health benefits and antioxidant activity of Portulaca oleracea L. International Journal of Community Medicine and Public Health, 11(5), 245–252.
  23. Twaj, A. M., Khan, S., & Qureshi, M. I. (2020a). Role of plant tissue culture in crop improvement and conservation. Journal of Applied Biology & Biotechnology, 8(4), 87–95.
  24. Twaj, A. M., Qrunfleh, I. M., & Abu-Romman, S. M. (2020b). Plant tissue culture as a tool for sustainable agriculture. Plant Cell Biotechnology and Molecular Biology, 21(25–26), 85–96.
  25. Wang, Z., Li, Y., Chang, S. X., et al. (2022). Effects of organic amendments on soil quality and crop yield: A global meta-analysis. Science of the Total Environment, 807, 150697.
  26. Xu, M., Zhao, Z., Fang, J., Li, P., & Yan, J. (2024). Somatic-cell regeneration and tissue culture of Portulaca oleracea L. Plants, 13(2), 456.
  27. Zang, H., Blagodatskaya, E., Wen, Y., et al. (2022). Soil microbial responses to organic amendments and implications for nutrient cycling. Soil Biology and Biochemistry, 165, 108528.

Authors

Nabaa Adnan Basheer AL-Taei

Rehab Abdul Jabbar Hamed Al-Bakr

Diar Najim Abdulla

Identifiers

DOI: 10.33899/jre.v4i2.64220

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