Inhibitory activity of licorice and Broccoli extracts on H.pylori bacteria

During the current study, both licorice roots and broccoli fruits were taken from the local markets of the city of Mosul for the purpose of separating and identifying some active compounds, which included the use of the polar solvent ethanol for the purpose of liberating the phenolic compounds from the sugar compounds associated with them. The process of acid hydrolysis of the ethanolic extract was conducted and then these compounds were identified. Using high-performance liquid chromatography (HPLC) technology.

From a biological standpoint, plant extracts of licorice roots and broccoli plants were used on H.pylori  bacteria, which were identified through molecular and biochemical methods to verify the effect of these extracts on bacteria through the inhibition of bacterial growth in dishes.

On the other hand, the inhibitory effect of licorice root extract and broccoli on H.pylori  bacteria varied. In the ethanolic extract of licorice, the highest diameter of inhibition was 13 mm at a concentration of 200 mg/mm, while the lowest diameter of inhibition of bacterial growth was 4 mm at a concentration of 25 mg/mm. The ethanolic extract of broccoli plants had the highest inhibition diameter of 8 mm at a concentration of 200 mg/mm and the lowest diameter of inhibition was 4 mm at a concentration of 100 mg/mm. Also, the results of the antibiotics that were used during the current study varied, as the highest diameter of inhibition was 20 mm for the antibiotic Ciprofloxacin, while it was less The diameter of inhibition was 3 mm for the antibiotic Cefotaxine.